The synthesis of antiviral ␤-chemokines has joined cytolysis as a potential mechanism for the control of HIV-1 infection by CD8 ؉ T cells. Recent evidence suggests that these two effector functions can diverge in some individuals infected with HIV-1; however, little is known about the CD8 ؉ T cell subsets in normal individuals that synthesize antiviral ␤-chemokines. In this report, we have used mutliparameter flow cytometry to characterize the T cell subsets that secrete the antiviral ␤-chemokine macrophage inflammatory protein (