Soil organic carbon (soil C) sequestration in forests is often higher under nitrogen (N 2 )-fixing than under non-N 2 -fixing tree species. Here, we examined whether soil C could be increased using mixed-species plantations compared to monocultures, which are less productive aboveground than mixtures. In addition, we compared soil C sequestration under N 2 -fixing trees with non-N 2 -fixing trees that received N fertilizer. Monocultures of Eucalyptus globulus (E) and the N 2 -fixing Acacia mearnsii (A) and mixtures of these species were planted in a replacement series: 100%E, 75%E + 25%A, 50%E + 50%A, 25%E + 75%A, and 100%A. Soil samples were also collected from fertilized monoculture treatments (100%EFer) of E. globulus (250 kg N ha -1 ).Total organic C, N and phosphorus (P) were determined at age 8 years at two soil depths (0-10 cm and 10-30 cm) and three density fractions of soil organic matter (SOM) were quantified for 0-5 cm depth. Soil C was highest in the 50%E + 50%A mixed stand and was highly correlated with aboveground biomass, not to the percentage of A. mearnsii in mixtures. This was largely due to soil C at 10-30 cm because there were no treatment effects on soil C at 0-10 cm. All density fractions of SOM at 0-5 cm increased with the percentage of A. mearnsii. In E. globulus monocultures, N fertilization did not increase soil C when compared with unfertilized stands.These results indicate that the inclusion of N 2 -fixing trees into eucalypt plantations may increase soil C stocks through increased productivity.
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