No abstract

Reactive oxygen species (ROS) production is one of the earliest responses when plants percept pathogens and acts as antimicrobials to block pathogen entry. However, whether and how pathogens tolerate ROS stress remains elusive. Here, we report the chromatin remodeling in Verticillium dahliae, a soil-borne pathogenic fungus that causes vascular wilts of a wide range of plants, facilitates the DNA damage repair in response to plant ROS stress. We identified VdDpb4, encoding a histone-fold protein of the ISW2 chromatin remodeling complex in V. dahliae, is a virulence gene. The reduced virulence in wild type Arabidopsis plants arising from VdDpb4 deletion was impaired in the rbohd mutant plants that did not produce ROS. Further characterization of VdDpb4 and its interacting protein, VdIsw2, an ATP-dependent chromatin-remodeling factor, we show that while the depletion of VdIsw2 led to the decondensing of chromatin, the depletion of VdDpb4 resulted in a more compact chromatin structure and affected the VdIsw2-dependent transcriptional effect on gene expression, including genes involved in DNA damage repair. A knockout mutant of either VdDpb4 or VdIsw2 reduced the efficiency of DNA repair in the presence of DNA-damaging agents and virulence during plant infection. Together, our data demonstrate that VdDpb4 and VdIsw2 play roles in maintaining chromatin structure for positioning nucleosomes and transcription regulation, including genes involved in DNA repair in response to ROS stress during development and plant infection.

No abstract

2015): Prediction model of atmospheric refractive index structure parameter in coastal area, Journal of Modern Optics,In this paper, we focus on the prediction of atmospheric refractive index structure parameter (C 2 n ) in coastal area using the routine meteorological parameters. Based on the micrometeorology, macrometeorology and Monin-Obukhov similarity theory, three modified prediction models of C 2 n are presented in combination with the long-term observation data of C 2 n and meteorological parameters in coastal city, respectively. For different weather, the applicable cases of three C 2 n prediction models are comparatively analysed and their applicable effects are comprehensively evaluated. The results indicate that the modified micrometeorology model of C 2 n shows better applicability for overcast sky, the offshore macrometeorology model of C 2 n displays better predictability for sunny day and the offshore Thiermann model provides better availability for overcast sky, cloudy day, overcast to sunny or sunny to overcast day.