in this paper, a class of the PCI data acquisition card using the professional interface chip PCI9054 and FPGA was designed, and the read and write transfer of PCI bus protocol were analyzed and implemented. Firstly, the architecture of system was proposed based on analysis of present design. Then, the detail design procedures are given, especially the logic control module and the SDRAM controller module. Its used the algorithmic state machine for the design of the modules in the FPGA. The ASM is the advanced state machine in present, and its worth to study. This paper used the hardware language to finish the entire programmer in the FPGA. The simulation results shown that the design proposed in this paper performed well.