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PNIPAAm gels undergo analogous collapse transitions in aqueous media. Recently the properties of PNIPAAm in aqueous media have attracted attention because of its scientific interest and applications [1][2][3]. In these studies of PNIPAAm, the form of hydrogel, microgel, lateices, membrane, thin film, coating and fibers are often applied. Although PNIPAAm are wellstudied, the applications of PNIPAAm were few. On the other hand, PNIPAAm are constantly copolymerized with other monomers and/or conjugate with biological molecules due to its weak mechanic ABSTRACT Poly N-isopropylacrylamide (PNIPAAm) is a well-known temperature-sensitive polymer. When the temperature is higher than the lower critical solution temperature (LCST), PNIPAAm aquous solution is cloudy (phase separation occurred). In contrast, when the temperature is lower than the LCST, PNIPAAm is soluble in water (a homogeneous solution). The lower critical solution temperature (LCST) in aqueous solution of PNIPAAm was about 32~33 . We prepared nano-scaled PNIPAAm particles containing carboxylic group on their surfaces by introducing acrylic acid monomer. The carboxylic groups were applied to conjugate with the amino group of the CD34 antibody. This immuno-conjugate can be applied on targeting the human CD34 positive cells, peripheral blood progenitor cells included, for cell purification and drug controlled release. In order