Orthodenticlehomeobox 1 (OTX1) overexpression had previously been associated with the progression of several tumors. The present study aimed to determine the expression and role of OTX1 in human hepatocellular carcinoma (HCC). The expression level of OTX1 was examined by quantitative real-time PCR (qRT-PCR) in 10 samples of HCC and paired adjacent non-cancerous tissues, and by immunohistochemistry (IHC) analysis in 128 HCC samples and matched controls. The relationship between OTX1 expression and the clinicopathological features werealso analyzed. Furthermore, the effects of OTX1 knockdown on cell proliferation and migration were determined in HCC cell lines. Axenograft mouse model was also established to investigate the role of OTX1 in HCC tumor growth. TheqRT-PCR and IHC analyses revealed that OTX1 was significantly elevated in HCC tissues compared with the paired non-cancerous controls. Expression of OTX1 was positively correlated with nodal metastasis status (P = 0.009) and TNM staging (P = 0.001) in HCC tissues. In addition, knockdown of OTX1 by shRNA significantly inhibited the proliferation and migration, and induced cell cycle arrest in S phase in vitro. Tumor growth was markedly inhibited by OTX1 silencing in the xenograft. Moreover, OTX1 silencing was causable for the decreased phosphorylation level of ERK/MAPK signaling. In conclusion, OTX1 contributes to HCC progression possibly by regulation of ERK/MAPK pathway. OTX1 may be a novel target for molecular therapy towards HCC.

The determination of the number of cavities is a very important part of the injection mold design process. In this paper, various factors influencing the number of cavities are analyzed comprehensively, a variety of methods are introduced for the calculation of the number of cavities, and the optimization principles for the reasonable choice of the number of cavities are proposed, for the reference of mold designers

From the basic concept of knowledge unit, with the efficient combination of axiomatic design and knowledge unit, using the hot runner system design of injection mold as the carrier, this article presents an information modeling method for concept product based on axiomatic design and knowledge unit as well as establishes corresponding model.

Concerning the properties of multi-specification and small-batch mechanical production, the relationship between the cost of instance module and the initial quotation of requirement product is analysed. The quotation rule mapping between the quotation formula and the request-characteristic variable as well as the product module is obtained based on the feedback amendatory coefficient, and the quotation method based on feedback amendment for multi-specification and small-batch mechanical production is realized. This method is adopted in the quotation of electrostatic precipitator and is confirmed to be effective.

The high-speed steel (HSS) reinforced by in-situ VC /35CrMo compound roll was produced by electromagnetic semicontinuous casting. The element distribution and the structure morphology of the interface were analysed, and the mechanical properties were tested. The results indicate that the thickness of diffusion layer is around 50μm. The microstructure of the diffusion layer is pearlite, and next to the diffusion layer, the microstructures are ferrite and pearlite at the alloyed steel side. There are in-situ VC particles in martensitic matrix. At the high-speed steel side. The microhardness of HSS and 35CrMo is about 700HV and 250HV, respectively, and that of diffusion layer is near 350HV, which is between that of HSS and 35CrMo. The impact toughness is 100 Jcm-2. On the basis of the experiment, the interface of compound roll prepared by electromagnetic semicontinuous casting obtains good microscopic structure and mechanical properties, which are the results of the metallurgically bonded interface.