The long non-coding RNA taurine up-regulated gene 1 (TUG1) has been shown to be dysregulated in various types of malignant cancer; however, its underlying mechanism of action has not been fully elucidated. The present study aimed to investigate the biological role and clinical significance of TUG1 in the progression of colorectal cancer (CRC). A reverse transcription-quantitative polymerase chain reaction assay was used to evaluate TUG1 expression in tissues from patients with CRC. The effect of TUG1 on cell viability of CRC cells using MTT assay. The influence of TUG1 on tumorigenesis was monitored using an xenograft model. The status of the Wnt/β-catenin signaling pathway was evaluated using immunofluorescence, western blotting and luciferase reporter assays. The results demonstrated that the expression of TUG1 was positively associated with the pathological grade and clinical stage of CRC patients. Knockdown of TUG1 inhibited the proliferation of CRC cells and attenuated the activity of Wnt/β-catenin pathway in CRC cells. In addition, TUG1 knockdown inhibited the tumorigenicity in the CRC xenograft model, as well as the nuclear localization of β-catenin and downstream gene transcription. Taken together, the data of the present study highlighted the pivotal role of the TUG1-Wnt/β-catenin signaling pathway in CRC, which could be targeted to improve the therapeutic efficacy of CRC.

Multiple longitudinal unified times are spread out laterally by this paper through analysis on ancient Greek temples, ancient Romanesque arched architecture, Gothic churches, domed architecture and other western classical architectural forms in various historical periods, and five types of relations between architectural forms and structures are found out through summarization in combination with contemporary technological characteristics. The conclusion is that harmonic coexistence of technology and art can be truly achieved through architectural form creation on basis of respect to the laws of structural science and through active cooperation between architects and structural engineers.

The modes of structural tectonic methods are proposed and the mode of creative cooperation by architects and structural engineers in the conceptual design stage for giving full play to their respective advantages are encouraged for joint design of architectural forms that meet structural safety requirements by this paper through analysis on functions and positions of structures in the architectural form framework system.The key function of observing the principle of structural correctness in the modes of architectural form design based on structural safety requirements is made clear.

Different materials will offer different physical property and visual effects, where the building form is the result of adapting to the material and showing its effect. This paper made some suggestions on selecting and applying materials involved in building design in terms of structure stress, making technique and artistic appearance. This method is a return to the essentials of building, and a key direction for building innovation.

Starting from analyzing current land utilization in China, this paper tries to find the related problems and their causes. The approaches to the saving and intensification of land utilization are suggested in economic construction, urbanization, and cultivated land protection in respects of theory, technology and administrative policies, to offer effective support for rapid urbanization, modernization and marketing and safeguarding of land utilization.