In vitro 5-fluorocytosine sensitivity tests of strains were isolated from chromomycosis patients undergoing therapy. Tests were rountinely performed for 0.1, 1.0, 5.0 and 10.0 mug/ml 5-fluorocytosine concentrations. In two cases resistance could be demonstrated up to 60.0 and 100.0 mug/ml. Fungistatic activity of 5-fluorocytosine against the agents of chromomycosis was confirmed. Sensitivity tests are useful to detect resistance which may occur during treatment. They are also helpful for guiding the clinician in the establishment of new therapeutic schedules.

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F o ram realiza d o s te s te s de s u sc e p tib ilid a d e "in v itr o " co m vá ria s a m o stra s de a g en tes, iso la d a s de p a c ie n te s com cro m o m ic o se fr e n te à 5 -flu o ro cito sin a e ao b u tü -sim p a to l (V a s c u la t) , n a s co n c en tra çõ MATERIAL E M ÉTODOSAs a m o s tra s fo ra m c o lh id a s de p a c ie n tes d a 2.a C lín ic a D e rm a to ló g ic a d a S a n ta Casa de M isericó rd ia de Belo H orizo n te, através de b iopsia. U m a p a r te d a m e sm a foi u tiliza d a p a r a estu d o s histo ló g ico s e outra p a r a estu d o s m icológicos.As a m o s tra s fo ra m c o lh id a s a n te s e d u r a n te o tr a t a m e n to com a s d ro g as 5--flu o ro c ito sin a e b u til-sim p a to l (V a scu lat). U m f ra g m e n to d a b ió p sia e ra tr a n s fe rid o p a r a o m e io d è S a b o u ra u d c o n te n d o a n t i b ió tico p a r a o iso la m e n to do fu n g o . U m a vez iso la d a , a a m o s tr a e r a re p ic a d a p a r a o m eio de S m ith , o n d e os fu n g o s so fria m u m a a d a p ta ç ã o , pois os te s te s fo ra m r e a liz ad o s n esse m eio. --s im p a tc l n a s m e sm a s c o n c e n tra ç õ e s f i n a is. A s p la c a s e ra m in c u b a d a s à te m p e r a t u r a a m b ie n te e a le itu r a e r a f e ita após u m a s e m a n a de c re scim en to . N a le itu r a * T ra b a lh o realizad o n o D e p a rta m e n to de M icrobiologia do I n s t i tu to de C iências B iológicas da U n i v ersidade F e d eral d e M inas G erais, d u r a n te a g estão do d ire to r P ro f. E d u a rd o O sório C isalpino.