No abstract

Objective: We aimed to investigate the vaccination status and the risk factors for the intensive care unit (ICU) support need of the laboratory-confirmed breakthrough COVID-19 infection inpatients. Materials and Methods: This multi-center point-prevalence study was conducted on inpatients, divided into two groups as ‘fully’ and ‘partially’ vaccinated according to COVID-19 vaccination status. Results: Totally 516 patients were included in the study. The median age was 65 (55-77), and 53.5% (n=276) of the patients were male. Hypertension (41.9%, n=216), diabetes mellitus (DM) (31.8%, n=164), and coronary artery disease (CAD) (16.3%, n=84) were the predominant comorbidities. Patients were divided into two groups ICU (n=196) and non-ICU (n=301). Hypertension (p=0.026), DM (p=0.048), and congestive heart failure (CHF) (p=0.005) were significantly higher in ICU patients and the median age was younger among non-ICU patients (p=0.033). Of patients, 16.9% (n=87) were fully vaccinated, and this group’s need for ICU support was statistically significantly lower (p=0.021). Conclusion: We conclude that older age, hypertension, DM, CHF, and being partially vaccinated were associated with the need for ICU support. Therefore, all countries should continuously monitor post-vaccination breakthrough COVID-19 infections to determine the national booster vaccine administration approach that will provide vulnerable individuals the highest protection. Keywords: COVID-19 vaccine, intensive care unit, risk factor, SARS-CoV-2 infection, vaccine breakthrough infections, variant of concerns

Metisilin dirençli Staphylococcus aureus (MRSA) hem toplum hem de hastane kökenli infeksiyonlardan izole edilen önemli bir patojendir. S.aureus suşlarında metisilin direncinin doğru ve hızlı bir şekilde belirlenmesi, infeksiyon kontrol önlemlerinde ve hastanede yayılımın önlenmesi açısından anahtar rol oynamaktadır. Akdeniz Üniversitesi Merkez Laboratuvarı'na gönderilen klinik örneklerden izole edilen toplam 163 S.aureus suşunun tanımlanması Gram boyama, katalaz, lam ve tüp koagülaz yöntemleri ve BD Phoenix otomatize identifikasyon ve antimikrobiyal duyarlılık sistemi kullanılarak yapılmıştır. Suşlarda nuc ve mecA genlerinin varlığı ve 16S RNA, özgül primerler kullanılarak polimeraz zincir reaksiyonu (PZR) yöntemiyle saptanmıştır. S.aureus olarak tanımlanan tüm suşların MRSA ID kromojenik agar besiyerine ekimi yapılarak plaklar 35°C'de aerobik ortamda inkübe edilmiş ve sonuçlar koloni renklerine göre değerlendirilmiştir. Bu çalışmada MRSA ID besiyerinin performansının değerlendirilmesi amaçlanmıştır.