The aim of this investigation was to determine serum levels of vitamin A, E, beta carotene, glutathione peroxidase (GSHPx), lipid peroxidation (MDA) and biochemical and haematological parameters during enflurane anaesthetised dogs. Ten kangal dogs were used and all animals were anaesthetised with enflurane for two hours and blood samples were taken before and 30, 120 minutes, 24 hours and 7 days during the anaesthesia. Vitamin E and beta carotene content were significantly (p<0.05 and p<0.01) higher before anaesthesia than after whereas serum GSHPx activity was not statistically different. However, serum levels of vitamin A and MDA were significantly (p<0.05) increased during the anaesthesia. In general, serum levels of aspartate aminotransferase, alanine aminotransferase, albumin, glucose, urea and creatinine were significantly (p<0.05 and p<0.01) increased during anaesthesia and returned to near normal values after 7 days of anaesthesia, whereas the white blood cell count was significantly (p<0.05 and p<0.01) decreased during the anaesthesia. However, the red blood cell count, haemoglobin and packed cell volume values, and levels of total cholesterol, triglycerides, total protein and globulin were apparently not influenced by the anaesthesia. In conclusion, we observed that the serum level of vitamin E and beta carotene were significantly decreased, whereas serum MDA and vitamin A levels were significantly increased during the enflurane anaesthesia.

IntroductionSkin wounds have long healing processes, and therefore they decrease the life quality of patients. The healing of wounds in a short time without any infections or scars is the desired outcome. In recent years, there have been many studies conducted on alternative treatment methods for wound healing (1-4). The studies related to wound healing aim to achieve wound healing without scar formation as well as accelerating the wound healing (4). However, studies are still ongoing to achieve optimal wound healing.From ancient times, herbal drugs have been used to treat wounds, and in many cultures their uses continue in traditional medicine. Historically, all medicinal preparations were derived from plants. Today, a significant number of drugs are developed from plants that are active against a number of diseases (5).Nigella sativa (NS) extract and its oil have antineoplastic, antibacterial, antifungal, anthelmintic, antidiabetic, and antioxidant effects, and they stimulate the immune system. These effects stem from the active compounds in the contents of NS. There are many active compounds in NS such as thymoquinone, dithymoquinone, thymol, carvacrol (volatile acids), nigellicine, nigellimine-N-oxide, nigellidine (alkaloids), and α-hedrin (triterpene) (6-8). It has been suggested that thymoquinone acts as an antioxidant and prevents membrane lipid peroxidation in tissues (9).Hypericum perforatum (HP) is used in various ways in wound healing. It is reported in the literature to have antispasmodic, sedative, antiseptic, and antidepressive effects as well as wound-healing effects (10,11). In addition, it is known to be effective against bacteria, viruses, inflammation, and pain (11,12).Today, traditional medicine is commonly used in wound healing. The aim of this study was to compare the effects of NS and HP in the form of an oil cream in concentration of 50% on wound healing in terms of clinical, biochemical, and histological aspects. Materials and methods ChemicalsNS, HP oil cream, and placebo cream were used. To prepare NS and HP oil creams, NS oil (Hel-Kim, 50 mL, Mersin, Turkey) and HP oil (Aksu Vital, 20 mL, İstanbul, Turkey) were separately mixed with equal amount of placebo cream.

No abstract

Bu olgu sunumunda, 4 günlük simental ırkı erkek bir buzağıda karşılaşılan üretral dilatasyon olgusunun klinik, radyografik, ultrasonografik tanısı ve tedavisi tanımlandı. Klinik muayenesinde disüri ve perineal bölgede içeriği idrar olan bir şişkinlik tespit edilen buzağının yapılan radyografik ve ultrasonografik muayenesi sonucu üretral dilatasyonu olduğu tespit edildi. Genel anestezi altında, şişkinlik bulunan bölge üzerinde 6 cm uzunluğunda bir ensizyon yapıldıktan sonra, deri katmanı geçilerek dilatasyonun olduğu bölgeye ulaşıldı. İdrarın boşaltılmasının ardından, üretranın dilate olan alanı daraltılarak üretrostomi işlemi yapıldı. Operasyon sonrası hayvana postoperatif olarak 7 gün parenteral antibiyotik uygulandı. Operasyonu takiben ilk hafta sadece üretrostomi yapılan bölgeden idrarını yapan buzağının sonraki süreçte idrarını penisten de yapmaya başladığı gözlendi. Bu sebeple üretrostomi ile oluşturulan açıklık kapatılarak hayvan kontrol altına alındı. Bir ay sonra hasta sahibi ile yapılan görüşmede hayvanın sağlık durumunun iyi olduğu öğrenildi.