Objectives: Observe the efficacy of vancomycin-loaded calcium sulfate（VCS）in promoting the natural healing of bone transport's docking site.Methods: We retrospectively analyzed 48 patients who used bone transfer to treat the tibia's infectious bone defects from 2013 to 2018. The patients were randomly divided into an experimental group receiving VCS treatment or a control group not receiving VCS treatment. After treatment, the two groups of docking site healing time, skin incarceration rate, natural healing rate, external fixation index, bony results, and functional results were compared.Results: The experimental group achieved bone healing in the docking site union for an average of (15.30±6.32) months after the operation. Among them, 25 cases healed spontaneously, two patients healed after bone cleaning and grafting; the average external fixation index was (2.10 ± 0.28) months/cm; Bone results: excellent in 19 cases, right in 7 patients, low in 1 case, functional derivatives: excellent in 16 points, good in 9 cases, and fair in 2 cases. The control group achieved bone healing at the docking site for an average of (15.29±5.36) months after the operation. Among them, 11 cases healed spontaneously at the docking site, and 10 cases of non-natural healing (6 points of skin incarceration) healed after cleaning and bone grafting; the average external fixation index was (2.09±0.25) months/cm; Bone results: excellent in 15 cases, right in 5 patients, fair in 1 case, functional products: excellent in 12 points, good in 8 cases, and fitting in 1 case. There was no significant difference in the healing time, bony results, functional results, and external fixation index between the two groups at the docking site (P>0.05). The experimental group's skin incarceration rate was lower than that of the control group, and the natural healing rate was higher than that of the control group (P<0.05).Conclusion: In bone removal surgery, the implantation of VCS in the infected bone defect area helps prevent the docking site skin incarceration and promotes the docking site's natural healing.