The leaves of Ocimum gratissimum and Vernonia amygdalina were analyzed for their chemical composition, minerals and vitamins. The results obtained from the analysis of the two plants were compared. The results revealed the presence of bioactive constituents comprising alkaloids (1.90 to 2.68 mg/100 g), tannins (0.04 to 0.07 mg/100g), saponins (0.06 to 2.94 mg/100 g), flavonoids (1.88 to 2.98 mg/100 g) and phenols (0.03 to 0.07 mg/100g). These plants contained ascorbic acid (241.06 to 310.62 mg/100 g), niacin (0.29 to 0.80 mg/100 g), riboflavin (0.08 to 0.09 mg/100 g) and thiamine (0.03 to 0.08 mg/100 g). These plants are good sources of minerals such as Ca, P, K, Mg, Na, Fe and Zn. The importance of these chemical constituents with respect to their roles in ethnomedicine in Nigeria was also discussed.

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The pollution of Ogbese River in Ovia NorthEast L.G.A. of Edo State, Nigeria was studied. Parameters like pH, temperature, electrical conductivity, colour, odour, chlorides, nitrates, phosphates and heavy metal ions such as lead, chromium, zinc etc, were analyzed. Also various techniques such as titrimetric methods, atomic absorption spectrophotometry were employed in the determination of these ions. The results show that most of the pollution indices of the water samples analyzed indicate that the Ogbese River water falls within the acceptable range for natural waters according to the World Health Organisation (WHO, 2006) standards. However, the Ogbese River water falls short of potable water on account of high presence of coliforms bacteria found in the river water. Defaecations of human, wild animals and runoffs from agricultural farmlands are believed to be the source of the coliforms bacteria.

The kinetic study of the reaction of Compound III of horseradish peroxidase with reduced nicotinamide adenine dinucleotide phosphate (NADPH) was investigated in phosphate buffer as a function of ionic strength at 25 °C. Two successive reactions involving increase and decrease in absorbance with time were observed. Each reaction was first order with respect to the concentration of horseradish peroxidase. The observed rate constants were ionic strength dependent within the range of 0.06 -0.30 M. The logarithmic values of the rate constants against the square root of the ionic strength showed that both NADPH and Compound III of horseradish peroxidase are of same ionic charges in the first reaction but different ionic charges in the second reaction.