During growth in a glucose‐tryptone‐mineral salts broth, B. thuringiensis HD‐1 Produced lactate, pyruvated, acetate, acetoin, and poly‐beta‐hydroxybutyrate (PHB). Based on the type and sequence of products found, all or part of the acetate appears to have been produced via the 2,3‐butanediol cycle. PHB was produced and utilized during spore formation and may have provided energy for that process.

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The effect of NaHCO, on the growth of Neisseria gonorrhoeae cultures was studied in a liquid and a semisolid growth medium. With a broth culture, NaHCO, (0.009 M) greatly reduced the lag phase and also increased the total growth. The same concentration of bicarbonate supported rapid growth when added to the semisolid medium if the plates were individually incubated in sealed plastic bags. In a container with a large air space, a higher concentration of NaHCO, was necessary to support growth. The assimilation of "4C-labeled NaHO, by growing cultures was also investigated.