The association between organizational culture and job satisfaction among employees of Colleges of Education was the focus of this paper. The study’s main objective was to determine whether certain aspects of organizational culture have an impact on workers’ job satisfaction. Employees of Ghanaian Colleges of Education were the focus of a cross-sectional study. A systematic questionnaire was used to collect the data; 200 questionnaires were given out, and 179 valid questionnaires were returned. Utilizing a stratified and straightforward random selection procedure, the personnel were chosen. The quantitative data was analysed using Statistical Product and Service Solution (SPSS), version 20.0. The correlation analysis was used to determine the relationships between organizational culture and job satisfaction. It emerged that except power culture, the other variables of the dimensions of organisational culture had a positive relationship with employees’ job satisfaction. In the light of this, the authors advise that the management and administrators of Colleges of Education should heavily emphasize the components of organizational culture which are authority, role, achievement, and support. The goal of this paper is to help employees understand the fundamentals of organizational culture, which will increase their job satisfaction. Keywords: Organisational Culture, Cultural Dimensions, Job Satisfaction, Colleges of Education, Employees