Donkeys have significant behaviour variations in comparison with horses, due to each species evolving in different environments. Donkeys have a reduced flight response compared with horses, but an increased tendency to display fight or freeze behaviours. Unlike horses, they can display territorial, defensive behaviour and show a wide range of sexual behaviours that require consideration during management. Donkeys can bond strongly to other donkeys or animals and separating bonded companions can cause stress severe enough to induce the potentially fatal condition hyperlipaemia. Donkeys show stoic body language in response to pain. Awareness of subtle behavioural indicators of fear and pain can improve donkey welfare and improve handler safety.