N6-methyladenosine (m6A) plays a critical role in the tumorigenesis of cervical cancer (CC). Here, we aimed to investigate the potential role of methyltransferase-like 3 (METTL3) in CC. Gene expression was determined via real-time quantitative polymerase chain reaction. Cellular functions were detected using colony formation, 5-ethynyl-2′-deoxyuridine (EdU), and Transwell assays. The interactions among METTL3, insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3), and apoptotic chromatin condensation inducer 1 (ACIN1) were confirmed using the MeRIP and RIP assays. An in vivo assay was performed to verify the role of METTL3 in CC development. METTL3 is overexpressed in CC, and therefore, its knockdown inhibits the proliferation and migration of CC cells. Silencing METTL3 inhibits tumor growth in vivo . Moreover, a positive association was observed between METTL3 and ACIN1. METTL3 interacts with IGF2BP3 to promote the mRNA stability of ACIN1, the overexpression of which induces the aggressiveness of CC cells. METTL3 promotes ACIN1 mRNA stability to accelerate CC progression, implying that METTL3 is a promising biomarker in CC.

Background: This protocol of systematic review aims to investigate the effectiveness of electrical stimulation (ES) on adverse events (AEs) caused by chemotherapy in patients with cervical cancer (CC). Methods: This systematic review of randomized controlled trials will be identified through searchers of PUBMED, PsycINFO, Scopus, Opengrey, Cochrane Central Register of Controlled Trials, EMBASE, Cumulative Index to Nursing and Allied Health Literature, Web of Science, Allied and Complementary Medicine Database, and Chinese Biomedical Literature Database. All the sources will be searched from the inception to the date of study search ran. Additionally, websites of clinical trials registry and reference lists provided in relevant studies and reviews will also be searched. Two independent reviewers will evaluate the eligibility criteria of all potential literature, extract the data, and determine the risk of bias for each included study. RevMan 5.3 software will be used to pool the data and to conduct a meta-analysis. Results: This systematic review will assess the effectiveness of ES on AEs caused by chemotherapy in patients with CC. Conclusion: The findings of this study may summarize the latest evidence for the ES on AEs following chemotherapy for CC. PROSPERO registration number: PROSPERO CRD42019120191.

Background: This study aims to explore the effect of melatonin for the management of endometriosis. Methods: We will search electronic databases (Cochrane Library, MEDLINE, EMBASE, CINAHL, Web of Science, Scopus, Allied and Complementary Medicine Database, Chinese Biomedical Literature Database, and China National Knowledge Infrastructure) from their inceptions to the February 29, 2020 without language and publication time limitations. The study identification, study quality assessment, and data extraction will be undertaken by two separate researchers. We will also appraise evidence quality of main outcomes by Grading of Recommendations Assessment Development and Evaluation, and statistical analysis performance by RevMan 5.3 Software. Results: This study will summarize up-to-date clinical evidence to investigate the effect of melatonin for the management of endometriosis. Conclusion: This study may provide helpful evidence of melatonin for the management of endometriosis. Systematic review registration: INPLASY202040093.

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Intra-partum fever usually complicates the delivery process, and its occurrence is often considered synonymous with chorioamnionitis. This inevitably leads to the use of antibiotics for the affected mother. A review of the literature suggests that this approach is not always appropriate. Most cases of intra-partum fever are secondary to non-infectious factors. Both infectious and non-infectious maternal fevers are associated with transient adverse neonatal complications, while maternal fever at delivery is an important risk factor for long-term neonatal developmental outcomes, including encephalopathy, cerebral palsy, and neonatal death. Also prenatal antibiotic exposure increases the risk of developing allergic diseases in children such as asthma and eosinophilic esophagitis. Timing of intrapartum antibiotic administration is very challenging. More research is needed to explore the etiology of intra-partum fever, to discover ways to prevent and control maternal hypothermia, and the proper use of intra-partum antibiotics to reduce unnecessary antibiotic exposure to the fetus. Therefore, the purpose of this study was to review the prenatal and intra-partum factors associated with fever during labor and delivery and to find ways to prevent and treat them to reduce maternal and fetal complications. Effective measures were taken for management to reduce maternal and fetal complications.