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We generated a genomic DNA library for Nothofagus solandri using 454 sequencing. Sequences from this library were screened for simple sequence repeat (microsatellite) motifs. Polymerase chain reaction (PCR) primer pairs were designed for 44 candidates and tested on a selection of samples collected from the wild. Seven of these PCR primer combinations produced interpretable polymorphic products. These have been assayed in 176 plants representing all the New Zealand species of Nothofagus subgenus Fuscospora (Nothofagus fusca, Nothofagus truncata and Nothofagus solandri). These markers will be applied to taxonomic, evolutionary and ecological studies of New Zealand beech.

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