South Africa is prone to droughts and is heavily dependent on stored water resources. Few studies in South Africa have investigated the impact of drought on water quality when rainfall resumes and reservoir sediments are rewet. Other research has shown that several negative impacts may occur with rewetting, including eutrophication, and acidification with increased metal solubility. In this study, the impact of drying and rewetting sediments on the mobility of nutrients and metals is explored using sediments from Loskop Reservoir. Rarely exposed sediments were collected from three sites, dried at 35°C for 36 days, and then rewet under aerobic conditions. Filtered water samples were collected on Days 1, 2 ,4, 7, 11 and 16 following rewetting, and analysed for , and were compared to reference (non-drought) treatments that were maintained under saturated conditions. Acute toxicity tests were performed using Daphnia magna to establish if toxic effects were associated with changes in water quality following sediment rewetting. Following rewetting, elevated SO 4 2-indicated the mobilisation of acid from sulfide oxidation, with an associated reduction in pH. Nitrification was evident as high quantities of organic N and NH 4 + were initially released, followed by increases in NO 2 -+ NO 3 -. Total P increased, but PO 4 3-reduced. This was attributed to complexes formed between PO 4 3-and Fe (FePO 4 ) and Al (AlPO 4 ). Amounts of Fe and Al released were lower following rewetting due to the formation of relatively immobile (hydr)oxides. In contrast, Mn increased substantially reaching maximum values > 2 300 mg/m 2 (14 000 µg/L). There were no acute toxic effects observed to D. magna. This study highlights the potential for acidification, mobilisation of nutrients, SO 4 2-, and Mn following rewetting of reservoir sediments under aerobic conditions. This information may be incorporated into reservoir and catchment management plans in the form of revised water quality guidelines and a better ability to predict how drought impacts water quality in our stored water resources.