Two experiments, a performance experiment and a mineral balance study, were conducted on grower-finisher pigs (42 to 101 kg live weight) to investigate the effects of Peniophora lycii phytase enzyme and 25-hydroxyvitamin D 3 (25-OHD 3 ) on growth performance, carcass characteristics, nutrient retention and excretion, and bone and blood parameters. The two experiments were designed as a 2 3 2 factorial (two levels of phytase and two levels of 25-OHD 3 ). The four diets were T1, low-phosphorous diet; T2, T1 1 phytase; T3, T1 1 25-OHD 3 and T4, T1 1 phytase 1 25-OHD 3 diet. In all, 25 mg of 25-OHD 3 was used to replace 1000 IU of vitamin D 3 in diets T3 and T4. Diets were pelleted (708C) and formulated to contain similar concentrations of energy (13.8 MJ DE/kg), lysine (9.5 g/kg) and digestible phosphorus (P; 1.8 g/kg). Neither the inclusion of phytase nor 25-OHD 3 in the diet had any effect on pig performance. There was an interaction between phytase and 25-OHD 3 on calcium (Ca) and P retention ( P , 0.01) and on the apparent digestibility of ash ( P , 0.01), P ( P , 0.001) and Ca ( P , 0.001). Pigs offered phytase diets only, had a higher retention of Ca and P and digestibility of ash ( P , 0.01), P ( P , 0.001) and Ca ( P , 0.01) compared with pigs offered unsupplemented diets. However, when the combination of phytase and 25-OHD 3 were offered, no effects were detected compared with 25-OHD 3 diets only. Pigs fed phytase diets had higher bone ash ( P , 0.01), bone P ( P , 0.01) and bone Ca ( P , 0.05) concentrations compared with pigs offered non-phytase diets. In conclusion, pigs offered phytase diets had a significantly increased bone ash, Ca and P than pigs offered unsupplemented phytase diets. However, there was no advantage to offering a combination of phytase and 25-OHD 3 on either bone strength or mineral status compared to offering these feed additives separately.