The wild Geratotropis species which occur in Japan consist of Vigna angularis var. nipponensis, V. nakashimae, V. riukiuensis and V. reflexo-pilosa. The main aim of the present study is to detect crosscompatibility of adzuki bean and rice bean with these wild relatives.The cross of V. nakashim-ae with adzuki bean was successful in both directions and viable seeds were produced, while the cross with rice bean was not successful. V. riukiuensis is cross-compatible with adzuki bean and rice bean only when V. riukiuensis is used as male parent and the hybrid seeds showed normal germination. The cross between V. reflexopilosa and the two cultigens adzuki bean and rice bean did not succeed in either direction.The present results suggest that V. nakashimae and V. riukiuensis could be used as gene sources for the adzuki bean breeding programme through interspecific hybridization. Successful hybrids between adzuki bean and rice bean cannot easily be produced. However, since V. riukiuensis is cross-compatible with both adzuki bean and rice bean, V. riukiuensis could be useful as a bridge species between adzuki bean and rice bean.
Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is the destructive pest of over 300 cultivated and wild fruits all over the world. Present study describes the morphology, essential oil content and composition and bioassay on fruit fly attractant ability of essential oil of two Ocimum tenuiflorum morphotypes (MT 1 purple and MT 2 purple-green) grown in Sri Lanka. Four months old, O. tenuiflorum aerial parts were hydro-distilled for 4 h. GC-MS analysis of essential oils and bio-assay for B. dorsalis attractant ability was performed using previously established methodologies. The yield of the essential oils of O. tenuiflorum MT 1 and MT 2 were 1.51±0.02% and 1.45±0.01% (v/w), respectively. Eighteen compounds were identified, which encountered over 97% of the oil constituents. The main constituents found in the oil of MT 1 were methyl eugenol (72.50±1.03%) followed by β-caryophyllene (17.53±2.0%), germacrene D (1.55±0.10%), β-elemene (2.46±0.17%), while methyl eugenol (64.23±2.43%), β-caryophyllene (13.29±2.18%), β-elemene (6.94±1.41%), germacrene D (2.47±0.96%), were from extracted from MT 2 . Bioassays conducted on essential oils of MT 1 , MT 2 and purified Methyl Eugenol demonstrated that the B. dorsalis attractant ability of essential oil MT1 (106±8.1), MT2 104±2 and commercial Methyl Eugenol (111±8.5) was not significantly different during the first week of the experiment. Results of our study open an avenue for use of essential oil of Ocimum tenuiflorum as potential natural para pheromone source for fruit fly control and monitoring in fruit industry in Sri Lanka.
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