The principle reason for the examination article is to assess the genoprotective action of the herbal source leaves extract of Indigofera tinctoria against insecticidal such as transfluthrin using Allium cepa as a biomarker. Indigofera tinctoria has the potential secondary metabolites, inorganic elements in phytochemical screening and different functional groups also. So that It possesses excellent anticancer, anti-inflammatory, anti-oxidant, antifungal, antibacterial, antitumor, anti mycobacterial, mosquitocidal, immunostimulatory properties. Normally for mutation, chemotherapy and radiation treatments are used. Here we used herbal remedy as Indigofera tinctoria against transfluthrin by using Allium cepa as a biomarker and the mitotic record and DNA discontinuity likewise determined. It contains high amount of secondary metabolites, inorganic elements and functional groups. The mitotic index remains exactly same at before and after treatment by calculating the number of roots and its length in segments like dividing and non-dividing cells. We concluded that Indigofera tinctoria repaired the mutation act against insecticidal transfluthrin. So it acts as an excellent mutant repair mechanism against insecticidal.