Summary:A new enzymatic method for the determination of cholesterol in serum and plasma was evaluated in 8 separate laboratories in comparison with routine and reference methods.Investigation of the analytical reliability in the 2-26 mmol/1 measurement ränge showed the following results:1. At the set reading points (10min at 25 °C and 5min at 37 °C) the reaction shows complete Substrate conversion. The colour complex is stable over a period of 60 min.2. The response to cholesterol is linear up to 26 mmol/1.3. Precision within the series was 0.6-2.8% in 20 determinations (coefficient of Variation).
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