Several natural products have been implemented as an alternative health care treatment and in discovery of effective modern drugs. A major focus of natural product chemistry has been toward drug design and discovery. Justicia adhatoda is a well-known Indian medicinal plant valued for its pharmacopeia. This plant root, bark, leaf and flower are used to heal several diseases and poisonous bites. The present work was to evaluate the phytochemicals and GC-MS analysis of J. adhatoda leaves extracts. The extracts were subjected to qualitative phytochemical screening using standard procedures. The result showed that the phytochemicals present in the extract of J.adhatoda are alkaloids, flavonoids, glycosides, cardiac glycosides, coumarins, hydroxy anthraquinones, tannins, phlobatannins, proteins, xantho protein, steroids and phenols. The GC-MS analysis of acetone extract showed the presence of many secondary metabolites like phytol (0.8%),  9,12,15-octadecatrienoic acid, (Z,Z,Z) (1.6%), butane, 2,2-dimethyl (0.21%), pentane, 2,3,3-trimethyl (0.22%), hexathiane (0.08%),  and benzenesulfonic acid (0.22%). The diversity of phytochemical present in the plant suggests that J. adhatoda could serve as a source of useful drugs. Keywords: Justicia adhatoda leaves, acetone extract, phytochemicals, GC-MS.