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4-, 5- and 6-Nitroindole have been investigated and compared with 3-nitropyrrole as universal bases in oligodeoxynucleotides. Of these the 5-nitroindole derivative was found to be superior giving higher duplex stability, and behaving indiscriminately towards each of the four natural bases in duplex melting experiments. 3-Nitropyrrole, whilst not discriminating between the natural bases, was found to lead to considerable destabilisation of the duplexes, particularly when multiple substitutions were made, in contrast to the 5-nitroindole nucleoside.

1965]Phosphate Esters : Efect of Changing Ester Grot@ 6559 and not to any peculiarity of the cyclohexyl ester as such. This general view has been confirmed by an investigation of the rates of hydrolysis of a series of esters of 2-hydroxypropyl phosphate (I). The esters were prepared by reaction between the alkyl or aryl phosphate and 1,2-epoxypropane in aqueous solution at about pH 8. The rates and product CHzO 0 -* The first-order rate constant for hydrolysis of barium propane-1,2-diol cyclic phosphate in 0 . 1~-NaOH a t 30" can be calculated as very roughly 3 x sec.-l from the data of T. Ukita, K. Nazasawa, and M. Irie. Pharm. Bull. (Tokyo), 1957, 5, 127. t The equation of the line for alkaline hydrolysis of the acetates is log koH = -0.26 pK, + 5-0 (cf. ref. 5 ) .D.

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