No abstract

No abstract

No abstract

In this report we describe the partial purification and characterization of an acid ribonuclease from beef brain nuclei (RNAase BN2). RNAase BN2 was purified approximately 85-fold. The optimum pH is 6.2 and the optimum temperature 55°C. The effect of ions on the RNAase BN2 and its K , were determined. RNAase BN2 is an endoribonuclease capable of hydrolysing polyA, polyU and polyC. Oligonucleotides produced by the hydrolysis of polyA by the RNAase BN2 have a monophosphate group at the 3' position.IT IS we11 known that Hn-RNA and pre-rRNA undergo a process of maturation in mammalian nuclei (PERRY, 1967;MADEN, 1970; WEINEIERG, 1973; DAR-NELL et al., 1973), involving partial degradation and cleavage of polynucleotide chains. There is some evidence that nuclear ribonucleases are involved in these phenomena (LIAU et al.

No abstract