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A two-generation female reproduction and teratology study was undertaking using 0, 1.5 and 3.0 ppm of T-2 toxin in a semisynthetic diet. Conception rate was lowest at the first breeding and lowest in the control group at this time, but in later breedings the conception rates of each group were very similar. No significant differences were noted in litter size, death of fetuses, sex of fetuses and gestational length. Feed consumption was similar in all groups, as were the non-pregnant or pregnant female weight gains. Although the second-generation offspring grew faster than the first-generation offspring, and significantly lower body weights were seen in the 3.0 ppm T-2 toxin group at 2, 5 and 6 weeks of age, all offspring reached the normal weight for CD-1 mice at 6 weeks of age. Gross and histological evaluation of all animals at post-mortem examination did not reveal any significant results, nor were there significant differences in major and minor or retardation defects among the treatment groups. Relative spleen weights of the second-generation offspring were significantly higher in control females compared with the treated groups but treated males had significantly increased relative spleen weights compared with control males of this generation. It was concluded that no long-term reproductive and teratological effects of low-dose dietary T-2 toxin were found in this study.

T-2 toxin, HT-2 toxin, Diacetoxyscirpenol (DAS), Roridin A, Verrucarin A and 3-Acetyldeoxynivalenol(3-AcDON) were dissolved in dimethyl sulfoxide and applied topically to mice. For a mortality study, groups of 10-20 male mice received either 5, 10, 20, 30 or 40mg toxin/kg BW.Further, mixtures of trichothecenes were applied, e. g.: T-2 toxin + DAS + 3-AcDON; T-2 toxin + DAS; T-2 toxin + 3-AcDON; DAS + 3-AcDON. Animals were observed until death or until day 14 after application, and the mortality rates were established. To study progressive morphological changes, groups of 20 male mice received 40mg/kg BW of the individual toxins listed above, and mice were killed at 6, 12, 18 or 24 hrs after application.With respect to cumulative mortality, it was found that T-2 toxin was the most potent toxin, followed, in descending order, by DAS, Verrucarin A, HT-2 toxin and Roridin A. N o deaths occurred with 3-AcDON. The combination of T-2 toxin + DAS + 3-AcDON produced a greater effect than predicted. The early systemic effects were found to be in partial agreement only with the mortality study: HT-2 toxin and DAS appeared more toxic in the early stages than T-2 toxin. Verrucarin A and Roridin A did not complete the pattern, and 3-AcDON caused very little damage.It is concluded that topical application of various trichothecenes results in characteristic toxic effects, yet these occur at different times and to varying degrees. Mixtures of trichothecenes have a synergistic effect as compared to singular trichothecenes.