The study assessed the anti-oxidant and anti-bacterial effect of fabricated green zincoxide nanoparticles (ZONs) of Andrographis alata whole plant ethanol extract. Phyto-fabricated nanoparticles (NPs) were characterized, which shows and confirms the formation of Andrographis alata ZONs through absorbance at 370 nm in UV-Visible spectra, shape and size were flower/star-like bunches with variable nm sizes by SEM and HR-TEM. From XRD and SAED confirms the purity of crystallite nature and crystalline phase and FTIR shows the functional groups involved in reducing as well as capping agents in the synthesis. EDAX shows the percentage of Zinc as 70.54% and Oxygen as 20.18% which shows the presence of Zinc and Oxygen in the synthesized Andrographis alata ZONs. Particle size distribution was measured by DLS was 22nm and PDI value was 1.000. Synthesized Andrographis alata ZONs exhibits good DPPH radical scavenging activity with IC50 values of 51.11µg/mL and displays good antibacterial activity against gram positive (Gm+) Corynebacterium diphtheriea, Bacillus subtilis, Streptococcus pyogenes and Staphylococcus aureus; gram negative (Gm-) bacteria Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and Salmonella typhii than leaf extract. Overall, the outcomes exposed that phyto-fabricated Andrographis alata ZONs can be splendid approach to form biocompatible and stable agents with significant potential in biological applications.