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Absorption and fluorescence properties have been studied of a new fluorescence reagent - 7-oxo-7H-benzimidazo[2,1-a]benz[d,e]isoquinoline-9-sulphonyl chloride and the corresponding sulphonamides derived from aliphatic amines. The fluorescence intensity is temperature-independent in the range 0-35 °C and depends linearly on concentration of the sulphonamides in the range 6 . 10-9 to 1 . 10-6 mol l-1. TLC separation of the sulphonamides is good, the yields of elution being 95%, which allows application of the procedure to determination of the amines.