Approximately 48 medical institutions in South Korea own and use blood irradiators; however, the quality control of blood irradiators is insufficient. This study was conducted to establish quality control procedures for blood irradiators using an alanine dosimeter and to ensure reliability of dosimetry. For dosimetry using alanine dosimeters, measurements were performed using an alanine dosimeter jig developed in this study to ensure dose delivery system reliability. Furthermore, the dose and dose distribution were verified, and the uncertainty was evaluated.