The materials fermented with natural products have been used in a variety of cosmetic applications as a whitening, wrinkle and inflammatory agent. Prunus tomentosa fruits have been used as a traditional medicine for anti-oxidant and have been reported to possess anti-microbial properties. This study investigated the deramal bioactive properties of the Prunus tomentosa fruits fermented with Lactobacillus acidphilus (PTF). The antioxidant activity of the PTF was measured by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. The cytotoxicity of human dermal fibroblast cell, murine macrophage cell line RAW 264.7 and B16-F10 melanoma cells were evaluated by sulforhodamine B (SRB) assay. Inhibitory effect of the PTF on nitric oxide (NO) prodction was evaluated by Griess Reagent System. The collagen synthesis was evaluated by using procollagen type-IC peptide (PIP) EIA kit. The tyrosinase activity, inhibitory effect of elastase and tumor necrosis factor-α (TNF-α) induced matrixmelloproteinase-1 (MMP-1) were also evaluated. It found that the PTF showed antioxidant activity without showing significant cytotoxicity in tested cells. The PTF were downregulated NO production in LPS-stimulated macrophages with nocytotoxicity (p<0.05). The PTF significantly induced collagen synthesis from the skin fibroblasts (p<0.05) and also dramatically inhibited MMP-1 activity (p<0.05). The PTF were inhibited activity of elastase and dramatically decreased melanin synthesis with inhibition of tyrosinase activity (p<0.05) and didn’t show cytotoxicity in B16-F10 melanoma cells. Therefore, the PTF showed bioactive properties on anti-oxidant, whitening, skin elasticity and anti-wrinkle efficacy. The PTF may be an appropriate choice for cosmetic products that aim to provide dermal bioactive effects.