This study was aimed to demonstrate that the AmpF'STR 1 Identifiler 1 kit will reliably amplify DNA in a reaction volume of 10 ml. For comparison, the DNA samples were also amplified at the currect in-house PCR reaction volume (12.5 ml). Even though the results showed significant difference in the peak height intensities, the quality of the profiles produced in the reaction volume of 10 ml was similar to those produced in the reaction volume of 12.5 ml. The application of this reduced volume PCR amplification would represent an additional 20% cost saving on the reagents without compromising the quality of the profiles obtained.2015 Elsevier Ireland Ltd. All rights reserved.

A B S T R A C TTo reduce the DNA profiling time, DNA samples were amplified using AmpF'STR 1 Identifiler 1 kit at reduced PCR cycling time amplification method without addition of any enhancement buffers or enzymes. The results showed no significant difference in the quality of the profiles produced compared to those produced in the manufacturer recommended condition. This study contributed in saving almost 25% of the time needed for PCR amplification without compromising the quality of the profiles.