No abstract

The present study aimed to know if the food of Spalax leucodon Egyptiacus has a role in its resistance to cancer. In vitro cytotoxic effects of the main food of Spalax leucodon Egyptiacus as a daily meal that was Asphodelus microcarpus which planted in Marsa Matroh governorate Eldabaa region .We use two fractions of the extract of this plant (Ethyl Acetate &Precipitate), cell viability and cytotoxic activities were assessed using The sulforhodamine B( SRB) assay, IC50 values were estimated for the A375 melanoma. We revealed that the IC50 for the A375 melanoma was 83 ug/ml for Ethyl Acetate (EtOAc) fraction, 65ug/ml for Precipitate (PPT) fraction after 48 hours of treatment. Asphodelus microcarpus fraction has considerable cytotoxic effects on the human melanoma cell line , its show effect on cell line A375 human melanoma ,further studies are required to emphasizes the relation between resistant cancer in Spalax leucodon Egyptiacus and its main food.

Human coronavirus and several picornaviruses are responsible for worldwide epidemic outbreaks, thus representing a heavy burden to their hosts. Coronaviruses (CoVs), enveloped positive-sense RNA viruses, are characterized by club-like spikes that project from their surface, an unusually large RNA genome, and a unique replication strategy. Roots of Asphodelus microcarpus-which planted in Marsa Matroh Governorate El-Dabaa region is the main food of Spalax leucodon Egyptiacus which showed immunity to many diseases. This plant was mainly reported to have anthraquinone and its derivatives. Asphodelus microcarpus tuber was extracted using methanol 80% that can extract polar, semi-polar, and non-polar compounds.Subsequently the methanolic extract was fractionated to, ethyl acetate. The present work showed that significant antiviral activity of A.microcarpus against coronavirus 229E, further studies are required to emphasizes this effect.