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INTRODUCTION Medium chain fatty acids MCFAs are recognized for their health benefits. They are used in enteral and parenteral nutrition for a series of medical conditions of patients suffering low absorption of fats, poor digestion, and metabolic difficulties related to cystic fibrosis, Crohn s disease, colitis and enteritis 1, 2. MCFAs with 6 to 14 carbons are of high interest for green chemistry due to their lathering and low-viscosity properties. They are widely used in detergents and lubricants. After esterification with alcohol, they can also be used as emulsifiers for foods and cosmetics, solvents for flavours, surface treatment of food products and as well as readily digestible fat in high-energy diets 3. In lipid research, thin layer chromatography TLC is commonly used for a rapid lipid fractionation and fatty acids profile characterizations. Nowadays, TLC constitutes a basic tool for modern analytical chemistry because of its