INTRODUCTIONAge related hearing loss known as Presbycusis is a universal feature of mammalian ageing and refers to sensorineural hearing impairment in elderly individuals. Among community-dwelling elderly people, the prevalence of hearing loss is 24% in those aged 65-74 years and 40% among those who are 75 years of age. The Molecular mechanism underlying Age related hearing loss is about 24%. Along with heart problems, hypertension and arthritis the hearing impairment is one of the four leading chronic health conditions experienced by the elderly people. 1,2 It is characterised by an age dependent decline of auditory function associated with loss of sensory hair cells, spiral ganglion neurons and stria vascularis cells in the cochlea of inner ear.3 It is also associated with difficulty in speech discrimination and central auditory processing of information.Hearing loss occurs when the tiny hairs inside the ear are damaged or die. The hair cells do not re-grow, so most hearing loss is permanent. Stria vascularis is the highly vascular tissue in the lateral wall of the cochlea. The spiral or cochlear ganglion is the group of nerve cells that serve the sense of hearing by sending a representation of ABSTRACT Background: Age related hearing loss is an universal feature of mammalian ageing and refers to sensorineural hearing impairment in elderly individuals. It was reported that increased generation of free radicals during cellular metabolism plays a major role in age related disorders. Therefore, the occurrence of age related hearing loss depends mainly on antioxidant status of the body. Hence, this study was undertaken to investigate the status of Glutathione and Superoxide dismutase in age related hearing loss. Methods: This study was conducted after the approval from institutional ethical committee. The study group included 25 patients diagnosed for age related hearing loss between the age group of 55-80 years of either sex and 25 healthy age and sex matched individuals as controls. Estimation of Glutathione was done by DTNB method and Superoxide Dismutase using standard procedures. The data was analysed using One Way ANOVA. P value less than 0.05 will be considered the level of significance. Results:The results showed that, the Glutathione level in Hearing Loss patients was increased significantly (p=0.0001) as compared to normal controls. The SOD Activity has declined significantly (p=0.001) in Hearing Loss patients as compared to normal controls. Conclusion: We found an association between the level of Glutathione and Super Oxide Dismutase in age related hearing loss. Thus the serum Glutathione and Super Oxide Dismutase level can be used as a biomarker for the assessment of age related hearing loss.