Background To study the changes of whole blood cells and plasma proteins in donors after plateletpheresis with multiple donations. Materials and Methods From October 2015 to September 2019, 42 donors with a plateletpheresis interval of 14-16 days and more than 20 times were selected as the research subjects. The venous blood samples were collected from the first and the last screening before plateletpheresis. The result of last screening before plateletpheresis as the observation group, and the first as the control group. Then, the venous blood samples was detected. Results The whole blood cells and plasma proteins in donors after plateletpheresis changes within a normal range in the two groups. The PLT counts in the the observation group was 220.1±40.4 x109/L, which was no statistically significant compared with the change of 216.6±44.5 x109/L in the control group(P>0.05). The HGB in the the observation group was 142.8±10.2 g/L, which was no statistically significant compared with the change of 142.1±8.3g/L in the control group(P>0.05). The HCT in the the observation group was 43.50±3.2%, which was no statistically significant compared with the change of 44.1±2.8% in the control group(P>0.05). The serum TP levels in the the observation group was 70.4±4.7g/L, which was no statistically significant compared with the change of 69.0±4.8g/L in the control group(P>0.05). The serum ALB levels in the observation group was 46.3±2.3g/L, which was no statistically significant compared with the change of 45.8±2.3g/L in the control group(P>0.05). Conclusion There have no effect on the whole blood cells and plasma proteins in donors after plateletpheresis with multiple donations.