Nanoparticles size can make easier for drugs to enter and react quickly to the body.In this study, nanoparticles Carica papaya L. leaf were used and combined withchitosan 1%. The aim of this study was to measure the effectiveness of thecombination of nanoparticles from Carica papaya L. leaf and chitosan 1% againstfibroblasts in the tooth socket of Wistar rats. Carica papaya L. leaf extract wasmade by maceration method and converted into a concentration of 25%, 50%, 75%and 100%, then made into nanoparticles using the nanoemulsion method. Chitosan1% was made using Na-TPP solvent using the ionic gelation method with a ratio of2:1 and after that it was mixed with nanoparticles of Carica papaya L. leaf thenmade into a gel preparation. The ANOVA statistical test shows a significant numberof p <0.05, so it can be ignored that the increase in the number of fibroblast cellsproduced by the nano gel extract is 25%, 50%, 75% and 100%, its 138, 173, 177.4and 328. Thus, the combination of 100% Carica papaya L. leaf nanoparticle extractand 1% nanocitosan was the most effective in increasing fibroblast cells on Wistarrat tooth socket.