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Evanguedes Kalapothakis (2019) Complete mitochondrial genome sequence of Hypostomusfrancisci (Siluriformes: Loricariidae), Mitochondrial DNA Part B, 4:1, 155-157, ABSTRACTHypostomus francisci, commonly known as "cascudo", is a fish of ecological importance found in the upper São Francisco River, Minas Gerais, Brazil. Here we present the complete mitogenome (mtDNA) of H. francisci. The whole molecule is 16,541 bp long and contains 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes, and one control region (D-loop). All the PCGs in the H. francisci mtDNA use the standard ATG start codon, except for Cox1, which utilizes GTG. Seven of the 13 PCGs contain TAA stop codons, three contain a TAG, and three contain the incomplete stop codons TA À or TÀ. ARTICLE HISTORY

Leporinus elongatus is an important commercial fish found in the La Plata and Paraná River basins. Next-generation sequencing was used to sequence the mitochondrial DNA (mtDNA) of L . elongatus. The mtDNA was assembled using the CLC Workbench software v. 9.0 and subsequently aligned to other 10 complete fish mitochondrial sequences to enable phylogenetic analysis using MEGA 7.0. The complete mtDNA molecule had 16,784 bp and its GC content was 43%. The mtDNA structure was similar to that of other vertebrates: two ribosomal RNA, 22 transfer RNA, 13 protein-coding genes, and a D-loop region containing 1115 bp. Phylogenetic analysis yielded a tree with high bootstrap value that was coherent with the current phylogeny proposed for Characiformes.

Mitochondrial complete genome (mtDNA) of Hoplias intermedius, commonly known as "tra ırão" is presented. DNA from a muscle tissue sample was sequenced by next-generation sequencing. To corroborate the results, phylogenetic analysis was performed with MEGA6 to compare the complete mitogenome of H. intermedius with genomes of other Characiformes species. The mtDNA molecule was circular and 16,629 bp in length. Gene content included 22 tRNA genes, 2 rRNA genes, 13 protein-coding genes and a D-loop noncoding region. All genes had ATG as the start codon. Six genes carried complete stop codons of AGG (COI), TGA (ND6) and TAA (ND1, ATPase 8, ND4L and ND5), while seven genes had incomplete stop codons. Phylogenetic relationships with other Ostariophysi species placed H. intermedius together with H. malabaricus as a monophyletic group belonging to the order Characiformes.