Aim: This study aimed to assess the oral hygiene practices and oral health status of middle aged (45–55 years) women residing in rural areas of Ambala district (Haryana). Materials and Methods: In this cross-sectional study, 79 villages were selected using simple random sampling technique through an online randomizer. The study participants (780) were selected through convenience sampling and oral health status was (dental caries, periodontal status, prosthetic needs, and dental fluorosis) recorded on a self-structured format based on World Health Organization Criteria (WHO, 1997). Group comparisons were done by using Chi-square test for categorical data. Results: In the present study, 69.2% of the study participants used toothbrush and toothpaste for cleaning teeth. Women in the age group of 45–50 years had lower mean Decayed, Missing and Filled Teeth (7.80 ± 5.21) when compared with the age group of 51–55 years (9.77 ± 6.16). Ninety-eight percentage of the women were in need of dental prosthesis. Among the study participants, 56.5% had 4–5 mm loss of attachment (LOA) and 23.2% had 6–8 mm of LOA. Difference between the need for multiunit dental prosthesis among the age groups (45–50 years) and (51–55 years) was statistically significant ( P < 0.05). Conclusion: Thus middle-aged women residing in rural area have high oral care need; however, there is quite a gap between the need for dental care and the amount of dental care utilized.