The menace of drug resistance, bioavailability and drug delivery to the target sites has motivated researchers to search for new antimicrobial agents from medicinal plants and subsequently use them for the biosynthesis of silver nanoparticles for effective killing of bacteria challenging to kill using crude extracts. The biosynthesis of silver nanoparticles was done using aqueous extract (AQE) of E<i>uphorbia heterophylla</i>, while characterization and the killing rate of conjugated silver nanoparticles (CA_gNP_s) were carried out using standard methods. The maximum wavelength obtained for CA_gNP_s was 410.33 nm, while the size distribution was 237.8 d.nm. The Fourier Transform Infra-Red result showed O-H (3308.94 cm^-1), which is responsible for stabilising and reducing silver ions, while the Transmission Electron Microscopy revealed the presence of monodispersed spherical shapes CA_gNP_s. The Energy Dispersive Spectroscopy confirmed the presence of silver. There were reductions in the clinical bacterial isolates exposed to CA_gNP_s as the exposure time increased. <i>Escherichia coli</i> was killed between 6-7 h while<i> Salmonella typhimurium</i> was killed at the seven has the value of 0.00 log₁₀ CFU/ml was recorded respectively. However, there were increments in the populations of clinical bacterial isolates in control as the time of exposure increased. Therefore, the study suggests that the CA_gNP_s exhibit intense antimicrobial activity and the potential to be developed as an alternative agent to treat bacterial infections, curb multidrug-resistant bacterial infection, and promote speedy drug delivery to the target sites.