MicroRNAs (miRNAs) play a critical role in various biological processes through regulation of gene expression post-transcriptionally. Although miRNAs are involved in cell proliferation and differentiation in mammals, few reports regarding the effects of host miRNAs on macrophage activation and differentiation are available in birds. Here, we reported that gga-miR-200b-3p acts as a positive regulator, enhancing macrophage activation and differentiation using an avian model. We found that ectopic expression of gga-miR-200b-3p in HD11 cells enhances the amount of MHC-II-positive cells and promotes the expression of pro-inflammatory cytokines and that gga-miR-200b-3p directly targets monocyte to macrophage differentiation-associated (MMD). The inhibition of MMD by gga-miR-200b-3p enhances the activation and differentiation of HD11 cells and increases the expression of pro-inflammatory cytokines. Collectively, these findings highlight a crucial role of gga-miR-200b-3p in macrophage activation and differentiation in birds.

The explicit expression of orbital angular momentum (OAM) spiral spectrum of Laguerre-Gaussian (LG) beam propagating in anisotropic plasma turbulence is derived based on Rytov theory. The relationship between the detection probability of OAM and the crosstalk with the anisotropy parameters, topological charge, radial index, wavelength, beam width, receiving aperture, inner and outer scales are discussed in detail. In order to quantify the effect of plasma turbulence, we compared it with atmospheric turbulence based on restriction function. To optimize OAM detection probability, we also compared the effects of circular aperture method(CAM) with focusing mirror method(FMM) on the propagation characteristics of the spiral spectrum. The results show that even in the strong turbulence region of atmospheric turbulence, the radial dimension of LG beam in plasma turbulence is nearly twice that of atmospheric turbulence and the modes crosstalk has almost reached its upper bound. Besides, by comparing the two optimization methods, we found the ability to reduce crosstalk is related to the choice of beam width and receiving aperture. Therefore, we give a method to optimally select between the CAM and the FMM. We found the corresponding relationship and gave an analytical formula. The FMM has a greater ability to reduce crosstalk than the CAM when the beam width≥4cm and the aperture≥3cm. We quantified the effect of plasma turbulence and provide a guideline for the selection of beam width and receiving aperture under these two optimization methods. The research results may be helpful in the field of optical communication.

Attention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder that occurs in early childhood and can persist to adulthood. It can affect many aspects of a patient's daily life, so it is necessary to explore the mechanism and pathological alterations. For this purpose, we applied induced pluripotent stem cell (iPSC)-derived telencephalon organoids to recapitulate the alterations occurring in the early cerebral cortex of ADHD patients. We found that telencephalon organoids of ADHD showed less growth of layer structures than control-derived organoids. On day 35 of differentiation, the thinner cortex layer structures of ADHD-derived organoids contained more neurons than those of control-derived organoids. Furthermore, ADHD-derived organoids showed a decrease in cell proliferation during development from day 35 to 56. On day 56 of differentiation, there was a significant difference in the proportion of symmetric and asymmetric cell division between the ADHD and control groups. In addition, we observed increased cell apoptosis in ADHD during early development. These results show alterations in the characteristics of neural stem cells and the formation of layer structures, which might indicate key roles in the pathogenesis of ADHD. Our organoids exhibit the cortical developmental alterations observed in neuroimaging studies, providing an experimental foundation for understanding the pathological mechanisms of ADHD. Graphical Abstract

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In a free space optical communication system based on vortex beams, the effects of spread and crosstalk caused by atmospheric turbulence should not be ignored. The orbital angular momentum (OAM) spectrum of the signal based on elliptic Gaussian beam (EGB) after propagation through non-Kolmogorov turbulent atmosphere are deduced, and a theoretical model of the spiral spectrum of EGB propagating through turbulent atmosphere is obtained. Numerically calculated OAM modes detection and crosstalk probability under different ellipticity parameters. The results show that the ellipticity parameter has a significant impact on the OAM spectral distribution of EGB and the transmission characteristics after turbulent atmosphere. The selection of appropriate ellipticity parameter can correspondingly reduce the degradation and crosstalk caused by turbulent atmosphere. We also compared a Laguerre-Gaussian beam (LGB) with EGB and pointed out the advantages and limitations of these two kinds of beams. The research results may be useful in the field of short distance optical communication and OAM-based multiplex communication.