The incidence of colorectal cancer (CRC) is increasing, with 1,931,590 new colorectal cancer patients worldwide in 2020. Fusobacterium nucleatum (Fn) has been widely reported as a harmful microorganism that promotes the development of CRC. Fn was found to enhance proliferation and metastasis of CRC cells by regulating cellular glucose metabolism and amino acid metabolism in trials. In a previous study, phenyl lactic acid (PLA), a natural metabolite of various probiotic bacteria, was found to have the ability to inhibit Fn as an organic acid. Therefore, further in-depth analysis of the mechanism of action of PLA in weakening Fn invasion into tumor cells provides a new therapeutic avenue for exacerbating CRC problems caused by intestinal pathogenic bacteria.