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With the adjustment of the socio-economic structure and the acceleration of industrial transformation and upgrading, the contradiction between talent supply and demand has become prominent, and the problem of difficult employment and low employment quality for graduates has not been effectively alleviated. This article explores the innovative mode of talent cultivation for e-commerce majors in application-oriented colleges on the background of rapid development in the era of "Internet +".At the current stage, there are many problems of E-commerce talent cultivation in application-oriented colleges such as unclear talent cultivation goals, the lack of integration of disciplines in the curriculum, the lack of systematisms and comprehensiveness of practice teaching system, insufficient integration of teaching content and regional economic development and the lack of double qualified teachers, which resulting in low employment satisfaction, poor innovation consciousness and innovation ability, prominent contradiction between talent supply and demand of e-commerce graduates. In addition, the talent cultivation structure and cultivation quality that cannot meet the requirements of regional economic structural adjustment and industrial upgrading. Aiming at the above problem has carried on the research and exploration, this paper puts forward ideas and suggestions on the construction of e-commerce talents cultivation mode in application-oriented colleges from the aspects of orientation of cultivation objectives, the formulation of talent cultivation scheme and curriculum system, the construction of practical teaching system, the construction of double qualified teachers teaching team, as well as school-enterprise cooperation, which aims to explore a suitable and effective path of e-commerce talent cultivation in application-oriented colleges.