Mechanical properties of cells are indicators of biological potential, quality and presumptive pathological status. Mammalian oocytes are particularly important cells from the point of view of testing their fertilizing potential and performing in vitro fertilization. However, only occasionally are their mechanical parameters measured.A new method for deformation measurement of in vitro-matured porcine oocytes has been developed and presented in this study. The method was used for multiparametric characterization of gametes using a MEMS-type microcytometer and a new methodology for determining cell deformability. The novel chip design, principle of operation, and fabrication processes are described in the study. Using original analysis of microscopic images with the usage of dedicated software and data analysis algorithms, deformation parameters were determined. Results indicate the relationships between parameters under the influence of cell compression and show that the behaviour of oocytes during measurements depends on the morphological quality of the cumulus-oocyte complexes they originated from.
3D printing enables fast and relatively easy fabrication of various microfluidic structures including microvalves. A check microvalve is the simplest valve enabling control of the fluid flow in microchannels. Proper operation of the check valve is ensured by a movable element that tightens the valve seat during backward flow and enables free flow for forward pressure. Thus, knowledge of the mechanical properties of the movable element is crucial for optimal design and operation of the valve. In this paper, we present for the first time the results of investigations on basic mechanical properties of the building material used in multijet 3D printing. Specified mechanical properties were used in the design and fabrication of two types of check microvalve—with deflecting or hinge-fixed microflap—with 200 µm and 300 µm thickness. Results of numerical simulation and experimental data of the microflap deflection were obtained and compared. The valves were successfully 3D printed and characterised. Opening/closing characteristics of the microvalve for forward and backward pressures were determined. Thus, proper operation of the check microvalve so developed was confirmed.
This paper presents a full-featured microfluidic platform ensuring long-term culturing and behavioral analysis of the radically different biological micro-objects. The platform uses all-glass lab-chips and MEMS-based components providing dedicated micro-aquatic habitats for the cells, as well as their intentional disturbances on-chip. Specially developed software was implemented to characterize the micro-objects metrologically in terms of population growth and cells' size, shape, or migration activity. To date, the platform has been successfully applied for the culturing of freshwater microorganisms, fungi, cancer cells, and animal oocytes, showing their notable population growth, high mobility, and taxis mechanisms. For instance, circa 100% expansion of porcine oocytes cells, as well as nearly five-fold increase in E. gracilis population, has been achieved. These results are a good base to conduct further research on the platform versatile applications.
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