Dengue fever develops with modifications in lab reports beginning the third day. It can appear on the fifth day with values reestablished to typical by the eleventh day. The examination results are applicable within the portrayal of organic markers in the advancement of the infection. They may be utilized as markers for the most severe structures along these lines empowering early assistance with the adaption of helpful lead for explicit patients. Seventy-three patients were analyzed for dengue fever. More significant variation was found in disease courses for the aged in hemoglobin and platelet count values. However, there was no significant difference between groups for the other forms of the disease, and the values were similar through the evolution. During the disease course, lymphocytosis was observed in all states, especially in the under-old age group. In all groups, thrombocytopenia was observed, and an increased AST enzyme occurred at the beginning of the disease. Keywords: Arbovirus, Hyponatraemia, Thrombocytopenia etc.

Microbial contamination plays a massive role in spreading diseases globally. Bacterial contamination of gram- positive, spore-forming bacteria, gram-negative rods, and fungus was tested on Indian rupee notes of various denominations. Bacteria and fungus were identified on almost all of the cash notes that were analyzed. Overall, our findings indicated that banknotes are contaminated with pathogenic germs and intestinal microorganisms and could be a source of severe infection. The present study recommends that paper currency be converted into plastic currency notes to avoid infections. Keywords: Contamination, fomites, Indian currency notes, infectious diseases, bacteria, fungi.

Genome editing is a flourishing tool that tends to be a boon for scientists to change an organism's DNA. With genome editing technology, scientists can add, remove or alter DNA, resulting in the inactivation of target genes, acquisition of novel genetic traits, and correction of pathogenic gene mutations. Different approaches were developed to edit the genome; one among them is CRISP-Cas which was recently developed. CRISPR associated proteins (CRISPR/Cas) are genome editing systems originated from prokaryotes that have allowed researchers to identify, photograph, alter, and annotate specific DNA and RNA sequences in a variety of living cells. Since using CRISPR/Cas is of low cost with high efficiency and good repeatability with a short cycle, it is used globally for genome editing. In the review, we analysed the utilisation of CRISPR/Cas as therapeutics to cure deadly diseases such as AIDS, Hepatitis B and human papillomavirus infection. Since CRISPR/Cas is cost-effective genome editing, the challenges to be faced in future due to usage of CRISPR/Cas and the areas where it can be utilised were discussed briefly in this review. Key message: Genetic technology, genome editing, editing tool, CRISPR/Cas, cost-effective, therapeutics.

Patient blood management is a crucial area introduced in almost all medical specialities, such as cardiac surgery, anaesthesiology, and orthopaedic surgery, but the PBM is even more critical in emergency obstetric care. Obstetric haemorrhage is one of the most frequent causes of maternal demise. Due to many factors such as comorbidities and physiological changes, estimating blood loss and the precise demand for blood transfusion during pregnancy is difficult. Numerous guidelines are available to assess the need for blood transfusion; however, the developed countries form all those guidelines used in developing countries. As a result, we investigated the various forms of blood transfusion reactions during pregnancy. We also looked at the frequency and risk variables for postpartum blood transfusion responses. Since patient blood management plays a vital role in minimizing Obstetric haemorrhage, we have also reviewed the guidelines for blood transfusion in obstetrics. Keywords: Blood transfusion, Patient blood management, Obstetric haemorrhage, Blood transfusion reactions, morbidity.