Milk is a complete food containing high-quality protein, the only source of lactose, and an excellent source of calcium. Thus, milk is prone to adulteration and to combat this menace, in India, the government appoints food safety officers who carry out chemical examination of milk and milk products. Formalin is the only legally permitted preservative for milk and milk products, in India, meant for analytical purposes. Scientific reports suggest that formalin interferes in maintaining the compositional profile; since accuracy in analysis of milk components is very important, these changes can be problematic. The other preservatives that have been described in the literature include mercuric chloride, potassium dichromate, hydrogen peroxide, bronopol, and azidiol Most of these preservatives are bactericidal but either involve a health hazard or environmental hazard or are unsuitable for long-term storage. Hence, there is a need to uncover combinations of antibacterial agents and bacteriostatic antibiotics for preserving milk and milk products until analyzed.

An apparent solidification time (AST) test was developed for the detection of foreign fats and oils in milk fat. AST values at 18°C for buffalo and cow milk fats ranged from 2 min 30 s to 2 min 48 s and 2 min 56 s to 3 min 26 s, while for pig body fat, goat body fat and hydrogenated vegetable oils, AST values were 1 min 30 s, 0 min 40 s and 1 min 50 s, respectively. Vegetable oils yielded no AST values, suggesting that adulteration can be detected using the AST method in the case of some but not all possible adulterants.

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