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Introduction: Kaempferol (3, 5, 7-trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) is a natural fl avonoid belongs to a subcategory of fl avonol family. The kaempferol -hydrogenated soy phosphatidylcholine (HSPC) complex was obtained by refl uxing and freeze drying method. Ultra violet (UV) -visible spectrophotometric method has been developed for the determination of kaempferol in kaempferol -HSPC complex. Objective: A validated UV -visible spectrophotometric method for determination of kaempferol in Kaempferol -HSPC complex. Materials and Methods: The Kaempferol -HSPC complex (Phytosomes) were prepared by dissolving both kaempferol and HSPC in 1, 4 -dioxane for refl uxing up to 2 h and freeze dried. The spectrophotometric detection of kaempferol was done at absorption maximum (λ max ) of 365 nm and 265 nm using methanol as solvent. The developed method was validated as per ICH guidelines. Result: The kaempferol content in Kaempferol -HSPC complex was found to be 79.32% and 79.19% at 365 nm and 265 nm. Kaempferol demonstrated good linearity in concentration range of 2-12 μg/ml (r 2 > 0.99) at 365 nm and 2-14 μg/ml (r 2 > 0.99) at 265 nm. Precision and mean recoveries were found to be in the range of (%RSD 0.0957 and 0.0580) and (% RSD 0.1461 and 0.0959) and 99.70% and 91.85% at 365 nm and at 265 nm. Limit of detection and limit of quantifi cation were found to be (0.015 μg/ml and 0.0191 μg/ml) and (0.0457 μg/ml and 0.0579 μg/ml) respectively. Conclusion: The developed method was found to be simple, specifi c, economic, reliable, accurate, precise, reproducible and used as a quality control tool for analysis of Kaempferol.

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