. Biceps brachii muscle innervation zone location in healthy subjects using high-density surface electromyography. Int. J. Morphol., 29(2):347-352, 2011. SUMMARY:Among the sequelae of central nervous system injury is spasticity which is difficult to manage in rehabilitation. Current treatments consider the administration of the botulinum toxin. However, results have proved highly variable in terms of effectiveness, attributable to the distance between the injection site and the innervations-zone (IZ). High Density sEMG (HDsEMG) has been used to gather information about muscle physiology and for location of the IZ. The aim of this study was to characterize the IZ of the biceps brachii muscle in healthy subjects during isometric contraction. We assessed the biceps brachii muscle of twenty healthy subjects (n=20) using a 64 surface electrodes matrix. The average location of the IZ was 34% distal to the line between the cubital fossa and the anterior acromion.