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Introduction: the variation of microorganisms of the vaginal canal is reported in patients with the presence of modified cervicovaginal polyps from the city of Puebla, Mexico; describing the vaginal microbiota and pathogenic microorganisms in order to analyze the possible association of these with the formation of cervicovaginal polyps. Methods: the clinical impressions and samples obtained from the patients who attended the Department of Cell Biology of the Faculty of Medicine of the Benemérita Universidad Autónoma de Puebla were analyzed within the framework of the Early Cancer Detection (DOC) program of the years 2001 to 2019, to the taking of cervicovaginal exfoliative cytology (PAP). Results: Of the 2,671 patients who met the inclusion criteria when presenting with cervicovaginal polyp, 82.14% reported the presence of microorganisms, being cocoid flora, the most frequent microorganism found in patients with cervicovaginal polyps, thus, the need for more studies deep and extensive in Mexico, due to the null reviews reported in the scientific literature.