Dissotis thollonii is widely used in Cameroon for the treatment of typhoid fever, gastrointestinal disorders, inflammation, kidney diseases, pregnancy control and sinusitis. However there is lack of experimental data on its possible toxicity. The aim was to investigate acute and subchronic toxicity of the ethanol leaf extract of Dissotis thollonii in healthy Wistar rats. In acute toxicity tests, a single administration of the ethanolic leaf extract (5000 mg/kg) of Dissotis thollonii was given orally to 4 female rats. The general behavior, adverse effects, and mortality were recorded for up to 14 days post-treatment. On the 15 th day, the rats were weighed and euthanized for necropsy. In sub-chronic toxicity tests, the extract (18.28; 62.50; 250 and 1000