Using the M13 phage display, a series of 7‐ and 12‐mer peptides which interact with new sulfobetaine hydrogels are identified. Two peptides each from the 7‐ and 12‐mer peptide libraries bind to the new sulfobetaine hydrogels with high affinity compared to the wild‐type phage lacking a dedicated hydrogel binding peptide. This is the first report of peptides binding to zwitterionic sulfobetaine hydrogels and the study therefore opens up the pathway toward new phage or peptide/hydrogel hybrids with high application potential.